Part:BBa_J01010

Riboregulator Lock 1

m-fold file for the cr12 riboregulator

Biobricked version of Isaacs' riboregulator cis repressed lock, crR12.

Usage and Biology

This part was made from the riboregulator lock described by Isaacs et al. in their 2004 publication (see references).

Fabrication notes:

The part sequence contains a ribosome binding site with a short complementary sequence upstream. When this piece is transcribed into mRNA it forms a stem-loop structure which "locks" the ribosome binding site. This folded mRNA structure is known as the 'cis-repressed' part. Part is also available with a constituitively on promoter as BBa_J01060

Experimental Notes:

Isaacs documentation (see reference #1):

Sequence secondary structure forms a stem-loop with two dispersed "bulge" sites (formed by intentionally mismatched base pairings) which destabilize the stem-loop enough to allow the corresponding taR12 "key1" to interject itself and open the loop.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]